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ppd0039 crop seq car puro vector  (Addgene inc)


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    Structured Review

    Addgene inc ppd0039 crop seq car puro vector
    Ppd0039 Crop Seq Car Puro Vector, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/ppd0039+crop+seq+car+puro+vector/pGSTag+BimL+(T56A)+(Plasmid+%2324253)/pmc12545207-246-18-20
    Average 93 stars, based on 2 article reviews
    ppd0039 crop seq car puro vector - by Bioz Stars, 2026-10
    93/100 stars

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    Related Articles

    Selection:

    Article Title: Systematic discovery of CRISPR-boosted CAR T cell immunotherapies
    Article Snippet: This cloning yielded the pPD0010_CROP-seq-CAR-mCherry2 vector (Addgene, 242533), from which all CROP-seq-CAR variants were derived. .. For efficient selection of transduced cells, mCherry was replaced with the PAC antibiotic resistance gene, resulting in the pPD0039_CROP-seq-CAR-Puro vector (Addgene, 242532) used in most screens. .. To generate variants with different CARs, this vector was linearized by PCR using Q5 High-Fidelity 2X Master Mix (NEB, M0492L) and primers prCART0026 and prCART0028, which exclude the sequence for the CD19 scFv.

    Plasmid Preparation:

    Article Title: Systematic discovery of CRISPR-boosted CAR T cell immunotherapies
    Article Snippet: This cloning yielded the pPD0010_CROP-seq-CAR-mCherry2 vector (Addgene, 242533), from which all CROP-seq-CAR variants were derived. .. For efficient selection of transduced cells, mCherry was replaced with the PAC antibiotic resistance gene, resulting in the pPD0039_CROP-seq-CAR-Puro vector (Addgene, 242532) used in most screens. .. To generate variants with different CARs, this vector was linearized by PCR using Q5 High-Fidelity 2X Master Mix (NEB, M0492L) and primers prCART0026 and prCART0028, which exclude the sequence for the CD19 scFv.



    Similar Products

    ppd0039 crop seq car puro vector  (Addgene inc)
    93
    Addgene inc ppd0039 crop seq car puro vector
    Ppd0039 Crop Seq Car Puro Vector, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/ppd0039+crop+seq+car+puro+vector/pGSTag+BimL+(T56A)+(Plasmid+%2324253)/pmc12545207-246-18-20
    Average 93 stars, based on 1 article reviews
    ppd0039 crop seq car puro vector - by Bioz Stars, 2026-10
    93/100 stars
    		  Buy from Supplier

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